Part:BBa_K4249000:Design
The biosynthetic gene for ERG production from N.crassa that catalyzing the first enzymatic step
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Design Notes
During the experiment, we found that some regions of the promoter sequence templates are rich in GC residues, which tend to fold into complex secondary structures and might not melt during the annealing phase of the PCR cycle. Also, the primers used to amplify GC-rich regions often have a high capacity to form self- and cross-dimers and a strong tendency to fold into stem-loop structures that can impede the progress of the DNA polymerase along the template molecule. Therefore, instead of PCR method, we obtained the gene expression cassettes by enzyme digestion.
Source
The egt1 is gene coding sequence, that from Neurospora crassa, a filamentous fungus that produces ergothioneine.